Note:
1) Most time Blunt ligation will be used for cloning the synthesized sequences using either SmaI or EcoRV or HicII sites of these vectors. Please remember to incorparate Restriction Enzyme sites flanking your sequences for your downstream cloning purpose.
2) For cloning into other non-standard vectors, such as pET28a, pcDNA4/TO, pEGFP-C1 or pFastBac1, cloning fee ￥800-￥2000/subcloning will be applied dependes on the difficulty of the subcloning.
3) The PlasMapper web site automatically generates and annotates plasmid maps using only the plasmid DNA sequences as input.
載體測(cè)序引物:
All vectors could be sequenced using the M13 Forward and Reverse Primers:
1) M13 Forward (-41): 5'- CGC CAG GGT TTT CCC AGT CAC GAC
2) M13 Reverse (-48): 5'- AGC GGA TAA CAA TTT CAC ACA GGA
Other technical assistance, please call or email us. |
